This method has been applied successfully to the analysis of sugars in fruit juice. Monosaccharides as well as glycoprotein carbohydrates could be detected in the microg/ml-range. Therefore an additional NaOH post column reagent has been continuously pumped through a mixing chamber into the mobile phase to form carbohydrate anions resulting in improved detection limits. As carbohydrates dissolved and eluted with pure water are present in the neutral form they are not detectable with PAD in contrast to carbohydrate anions formed at high pH values. Nearly baseline separations have been obtained for the glycoprotein carbohydrates of sialic acid ( N-acetylneuraminic acid, NANA), N-acetylglucosamine (GlcNAc) and N-acetylgalactosamine (GalNAc). Carbohydrates have been separated on POLYSPHER(R)CH OH columns using pulsed amperometric detection (PAD) and UV detection (lambda =196 nm) in series and pure water as mobile phase.
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